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Project Progress

Our primary PSARC project investigates microorganisms deposited over 100,000 years ago in a Greenland glacier (GISP2) ice core. This ice core serves as a model for severe conditions, such as cold temperatures, nutrient and oxygen limitations, desiccation, pressure, etc. that could exist extra-terrestrially. Previous research demonstrated the existence of a large population of ultra-small cells about 1/10 the size of an E. coli cell. Currently we are characterizing two isolates representing new species, one designated Herminiimonas glaciei sp. nov. and the other Chryseobacterium greenlandensis sp. nov. Additional ongoing analyses of the ice core include comparing the microbial diversity at different depths to determine whether any specific organisms could be a biomarker for the deposition climate or geographic origin. In addition, we designed long-term, sensitive experiments to test whether microbial metabolism could occur at subfreezing temperatures and demonstrated that model organisms could release gas at the lowest temperature tested, minus 32 degrees C.

Additional findings from our research include discovering and characterizing a plasmid from one of our unique ultrasmall isolates and recombining this to construct a vector that could be useful for genetic studies of Gram-positive organisms. We also concluded a metagenomic study of genomic DNA extracted and amplified from subseafloor sediment showing that archaeal cells, rather than bacteria, comprise the majority of this population. One unifying theme in all of our research is the development of sensitive detection methods that will help examine samples with low biomass and augment the ability to search for the presence of life elsewhere. In addition to these future ramifications, our research is adding considerable knowledge about microbial diversity, new cultivation approaches, and insight into microbial metabolism at sub-freezing temperatures. Our research abstract was selected by the American Society for Microbiology for a press release and was publicized through general articles.