2005 Annual Science Report
University of Rhode Island Reporting | JUL 2004 – JUN 2005
Subsurface Biospheres
Project Summary
Members of our team at UNC Chapel Hill focus on molecular studies of deep subsurface communities
Project Progress
Members of our team at UNC Chapel Hill focus on molecular studies of deep subsurface communities. In year 7, we published the first molecular community analyses (16S rRNA genes) of organic-poor deep marine subsurface sediments. In collaboration with MBL team members, we also undertook what may be the first rRNA analyses of deep subsurface communities. These analyses allow us to identify the active members of deep subsurface communities (in contrast to DNA-based assays that combine signatures of active, inactive and dead prokaryotes). Our DNA-based studies of formation fluid from a sealed borehole near Costa Rica indicate that the fluid helps to support a diverse, partially chemolithotrophic community.
The WHOI members of our team continued research on biomarkers and subseafloor processes. With PSU team member C. House, we showed that archaeal communities in deeply buried sulfate-methane transition zones are dominated by two novel lineages that are distinct from methanotrophs in surface sediments and, unlike near-surface methanotrophs, do not assimilate carbon from methane. To further test our Year 6 hypothesis of microbial ethanogenesis and propanogenesis in subseafloor sediments, we are searching for potential abiotic pathways of hydrocarbon formation. We also undertook molecular isotopic studies of peridotite-based hydrothermal ecosystems with abundant chemolithoautotrophs. To date, we have also systematically identified biomarkers that distinguish more than 100 cultures of prokaryotes that collectively cover all major phyla of Bacteria and Archaea.
Members of our team at URI continued to develop and apply techniques for quantification of deep subsurface activities and ecosystem structure. We showed that a deep subseafloor ecosystem is a thermodynamic homeostat where sulfate reduction, iron reduction, methanogenesis and possibly methanotrophy are mutually dependent and produce biologically useful energy throughout a sediment column deposited over millions of years. We are refining our extremely sensitive tritium-based method for detection of life and applying it to deep subseafloor sediments and (in collaboration with IPTAI team members) to deep gold mine samples. We developed and tested (1) a robust new method for determination of subsurface methane concentrations, and (2) techniques for isolation of intact cells from sediments. Future application of the latter techniques will greatly improve analyses of subsurface life.
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PROJECT INVESTIGATORS:
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PROJECT MEMBERS:
John Hayes
Co-Investigator
Kai-Uwe Hinrichs
Co-Investigator
David Smith
Co-Investigator
Arthur Spivack
Co-Investigator
Andreas Teske
Co-Investigator
Wolfgang Bach
Collaborator
Bo Barker Jørgensen
Collaborator
Jennifer Biddle
Collaborator
Steven Cheong
Collaborator
Marcus Elvert
Collaborator
Verena Heuer
Collaborator
Christopher House
Collaborator
Fumio Inagaki
Collaborator
Mark Lever
Collaborator
Julius Lipp
Collaborator
James Miller
Collaborator
Scott Rutherford
Collaborator
Florence Schubotz
Collaborator
Roger Summons
Collaborator
Michael Twardowski
Collaborator
Jens Kallmeyer
Postdoc
Antje Lauer
Postdoc
Bruno Soffientino
Postdoc
Ketil Sorensen
Postdoc
Helen Sturt
Postdoc
April Pariseault
Research Staff
Edward Roggenstein
Research Staff
Robert Sand
Research Staff
Miranda Smith
Research Staff
Kristin Smith
Research Staff
Karen Lloyd
Doctoral Student
Guizhi Wang
Doctoral Student
Carly Blair
Graduate Student
Kristofer Carlson
Undergraduate Student
Kelly Hanks
Undergraduate Student
Kate Harris
Undergraduate Student
Daniela Kalhoefer
Undergraduate Student
Melinda Montano
Undergraduate Student
Ben Swanson
Undergraduate Student
Laura Wehrmann
Undergraduate Student
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RELATED OBJECTIVES:
Objective 2.1
Mars exploration
Objective 2.2
Outer Solar System exploration
Objective 4.1
Earth's early biosphere
Objective 4.3
Effects of extraterrestrial events upon the biosphere
Objective 5.2
Co-evolution of microbial communities
Objective 5.3
Biochemical adaptation to extreme environments
Objective 6.1
Environmental changes and the cycling of elements by the biota, communities, and ecosystems
Objective 7.1
Biosignatures to be sought in Solar System materials
Objective 7.2
Biosignatures to be sought in nearby planetary systems