2012 Annual Science Report
Massachusetts Institute of Technology Reporting | SEP 2011 – AUG 2012
Micro-RNAs as Phylogenetic Markers
Since the beginning of 2011, we have published 12 peer-reviewed papers centered on three themes. First, we have pioneered the use of microRNAs (miRNAs) – small 22 nucleotide non-coding RNAs – as phylogenetic characters, and have recently gained new insights into the relationships of vertebrates, arthropods, brachiopods, and flies. Second, we have noted that the number of miRNAs an organism possesses seems related to their relative morphological complexity such that more complex animals have many more miRNAs than simple animals. Third, in a recent Science paper (Erwin et al.2012) we summarized all of our molecular-clock work to date which strongly suggests that bilaterians have a fairly extensive cryptic Precambrian history, which when coupled with our miRNA work makes this missing record even more enigmatic, and the resolution of this paradox is the focus of our current and much of our future NASA-sponsored work.
During the last year we have published 7 papers using microRNAs to help elucidate phylogenetic relationships that have thus far proved difficult using standard molecular means. Questions addressed range from the position of turtles within the reptiles (Lyson et al. 2011) to the monophyly of brachiopods relative to phoronids (Sperling et al. 2011) to the phylogenetic position of tardigrades relative to other ecdysozoans (Campbell et al. 2011). We have further used miRNAs to help test hypotheses concerning the advent and maintenance of morphological complexity. We have shown that complex animals have many more miRNAs than simple animals (see Fig. 1), and that secondary morphological simplification is accompanied by secondary loss of miRNAs. In particular we showed that acoel flatworms, long a proxy for a “typical” Precambrian bilaterian animal as they were reconstructed as basal bilaterians with a relatively simple morphology, were in fact closely related to modern hemichordates and echinoderms (Philippe et al. 2011), and thus their simple morphology was secondarily derived through loss, including the loss of a centralized nervous system, blood vascular system, and kidney. Further, this secondary simplification was accompanied by a dramatic loss of miRNAs (see Fig. 1). Current work is focused on further testing this correlation between morphological simplicity/complexity and miRNA repertoire.
PROJECT MEMBERS:Kevin Peterson
RELATED OBJECTIVES:Objective 4.2
Production of complex life.